A process for papain (cistein papainase) extraction and purification from fresh latex obtained from unripped fruits of Carica papaya is described. Samples of fresh collected latex were suspended in citratephosphate buffer containing reducing agents whose concentration and application conditions were found experimentally. The extract was centrifugated and the clarified solution subjected to ammonium sulphate precipitation. The insoluble fraction was resuspended in water, desalted and concentrated by tangential filtration and, the remaining water removed by liophylization. The process allowed 80% recovery of the initial protein content and 65% of the original activity, the product obtained can be described as a white powder very soluble in water. All the steps can be scaled-up for commercial papain production. Keywords: Papain, protease, carica papaya.
|Translated title of the contribution||Design of an experimental process for the production of lyophilized papain|
|State||Published - 2008|
- Carica papaya