TY - CHAP
T1 - Qualitative and quantitative NMR approaches in blood serum lipidomics
AU - Barbosa, Banny Silva
AU - Martins, Lucas Gelain
AU - Costa, Tássia B.B.C.
AU - Cruz, Guilherme
AU - Tasic, Ljubica
N1 - Publisher Copyright:
© 2018, Springer Science+Business Media, LLC.
PY - 2018
Y1 - 2018
N2 - Nuclear magnetic resonance (NMR) spectroscopy in combination with chemometrics can be applied in the analysis of complex biological samples in many ways. For example, we can analyze lipids, elucidate their structures, determine their nutritional values, and determine their distribution in blood serum. As lipids are not soluble in water, they are transported in blood as lipid-rich self-assembled particles, divided into different density assemblies from high- to very-low-density lipoproteins (HDL to VLDL), or by combining with serum proteins, such as albumins (human serum albumins (HSA)). Therefore, serum lipids can be analyzed as they are using only a 1:1 (v/v) dilution with a buffer or deuterated water prior to analysis by applying 1H NMR or 1H NMR edited-by-diffusion techniques. Alternatively, lipids can be extracted from the serum using liquid partition equilibrium and then analyzed using liquid-state NMR techniques. Our chapter describes protocols that are used for extraction of blood serum lipids and their quantitative 1H NMR (1H qNMR) analysis in lipid extracts as well as 1H NMR edited by diffusion for direct blood serum lipid analysis.
AB - Nuclear magnetic resonance (NMR) spectroscopy in combination with chemometrics can be applied in the analysis of complex biological samples in many ways. For example, we can analyze lipids, elucidate their structures, determine their nutritional values, and determine their distribution in blood serum. As lipids are not soluble in water, they are transported in blood as lipid-rich self-assembled particles, divided into different density assemblies from high- to very-low-density lipoproteins (HDL to VLDL), or by combining with serum proteins, such as albumins (human serum albumins (HSA)). Therefore, serum lipids can be analyzed as they are using only a 1:1 (v/v) dilution with a buffer or deuterated water prior to analysis by applying 1H NMR or 1H NMR edited-by-diffusion techniques. Alternatively, lipids can be extracted from the serum using liquid partition equilibrium and then analyzed using liquid-state NMR techniques. Our chapter describes protocols that are used for extraction of blood serum lipids and their quantitative 1H NMR (1H qNMR) analysis in lipid extracts as well as 1H NMR edited by diffusion for direct blood serum lipid analysis.
KW - H NMR
KW - H NMR edited by diffusion
KW - H qNMR
KW - Blood serum lipids
KW - Lipidomics
UR - http://www.scopus.com/inward/record.url?scp=85041476676&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-7614-0_25
DO - 10.1007/978-1-4939-7614-0_25
M3 - Capítulo
C2 - 29380328
AN - SCOPUS:85041476676
T3 - Methods in Molecular Biology
SP - 365
EP - 379
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -